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Thanks & Summary

I wanted to thank everyone who made this internship possible for me! Thank you to NEVBD-TEC and the staff there including Emilie M Gray and Emily Mader. Thank you to Tadhgh Rainey for hosting me in Flemington! “Dyakuyu” or дякую to Jack Petersen for guiding me through the world of Aedes albopictus, Natular SC, and of course Ukrainian, Polish, etc. I also wanted to thank the rest of Hunterdon County Vector Control, Nick, Marty, and Lexi, for teaching me all about treating the river, gravid traps, light traps, etc! I truly enjoyed all aspects of this internship from lab work on mosquito larvae to black fly field work. I thought that I would give a quick summary of everything I learned this summer! I completed 4 bioassays on Aedes albopictus larvae with different concentrations of Natular SC in 250mL of water. We collected data to create Probit analysis curves using computer software to estimate an LD50 (ours averaged around 0.5 to 0.7 microliters/Liter). We then started taking these estimates outside to treat larger amounts of larval habitat (about 5L). I also gained knowledge on mosquito identification in larval form as well as adult form through microscope work. I created permanent microscope slides using mosquito larvae from our different trials to use as voucher specimens. I also experienced the daily life of mosquito and vector control including setting and collecting gravid traps with stink water, collecting light traps, and treating the river for black fly. I also was able to go to smaller creeks and streams throughout Hunterdon County to collect black fly samples throughout the county. I appreciate all of these experiences because I feel I was able to apply concepts from the classroom and have a better understanding of science applications in the real world. I will be returning to Villanova University at the end of this week to continue my undergraduate degree in Biochemistry, but I will now be able to take everything I learned throughout these ten weeks with me!

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Welcome to my Internship Blog!

 Hello! My name is Abby Golembiewski, and I am a biochemistry student at Villanova University. This summer I will be interning with the Hunterdon County Mosquito and Vector Control Program in Flemington, New Jersey. I am excited to gain experience in field and laboratory work while studying black flies and Asian tiger mosquitoes!!  

Week 4: (6/24-6/28)

  Black Fly:  This week, I did not do too much black fly work, but I was able to go to two small creeks to take samples from. However, at one of those sites we were unable to find any black fly (which is technically a good thing!). The other site was successful, so we added one more sample to the small creek collection. Below is a picture!! A lot of these black fly small creek spots are so pretty! Mosquitoes:  On the bioassay side of things this week, I first collected the 96 hour data from last Thursday. There were some interesting results, and when the Probit analysis was run, it said that our LD50 was 0.08 microliters/Liter. This is similar to the results we received last time (0.05 microliters/Liter). We then decided to start our first outdoor trial and scaled up to a “mesocosm” (5 L of larval water rather than 250 mL). We put 5 mL of 1 microliter/Liter solution into it. I took note that most of the larvae were dead within the first 24 hours, and they were all dead after 72 hours

Week 1 (6/3-6/7):

  During my first week as an intern, I got a taste for both lab and field work!! In the lab, I first learned common types of mosquitoes including the Asian Tiger mosquito or Aedes Albopictus, which we will be working with over the course of the summer. I worked with “Dr. Jack” Petersen a lot this week, and he was incredibly informative on all things mosquitoes! I was very excited to get started, and we set up a new infirmary as well as a sample bioassay on the first day. I already feel more confident in my ability to determine different life stages of the mosquitoes. Additionally, much of the first week was defining the first bioassay we are going to be running with Natular SC, which is a larvicide that would be new to New Jersey.  Our research question is how many microliters of Natular SC are needed to treat 100 L of larvae? We hope to answer the question through a regression and probit analysis of the data. We then decided 8 different concentrations to use for 8 different treatments