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Week 6: (7/8-7/12)

This past week was very busy but very exciting! I was able to attend a field trial on Tuesday of this week in Mercer County for a new product from Valent BioSciences. This experience was awesome, and I learned a lot! When I first got there, I helped to make 200 post treatment cups to put mosquitoes from different reps of the trial in. Then, I aspirated mosquitoes for the first time! We separated mosquitoes (albopictus, pipiens, and field collected mosquitoes) from different counties into “tambourines” for three different distances from the sprayers and three different trials. There were also tambourines that acted as controls for the trial. At first, aspirating mosquitoes was a little difficult for me, but then I got the hang of it. Then, we waited for the weather. At 9:30, the trial was ready to start since the ground temperature was cooler than higher in the air. After each rep, I helped to aspirate the mosquitoes from the tambourines into the post treatment cups that we created earl
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Week 5: (7/1-7/5)

Happy July!  Although this week was short with the holiday weekend, I was still able to get a lot done as well as create a game plan for the coming weeks!  Mosquitoes:  The results of last week's mesocosm study in a 5 L container was that the pupae lived but the larvae died. This was expected as fourth instars and pupae do not feed and therefore would not be impacted by the larvicide. I also started another mesocosm study this time with 2 5L buckets. The treatment was 1 microliter per liter. The larvae in each bucket were taken from the two different colonies that we have which is important for what we learned later in the week.  This week I was able to do a lot of microscope work, which was really interesting for me. I looked at both live and dead larvae. I also learned the process of making permanent microscope slides which involved heat shocking the live larvae, then placing them in 80% ethanol to kill them, and lastly, 100% ethanol to dehydrate them. I learned the key identific

Week 4: (6/24-6/28)

  Black Fly:  This week, I did not do too much black fly work, but I was able to go to two small creeks to take samples from. However, at one of those sites we were unable to find any black fly (which is technically a good thing!). The other site was successful, so we added one more sample to the small creek collection. Below is a picture!! A lot of these black fly small creek spots are so pretty! Mosquitoes:  On the bioassay side of things this week, I first collected the 96 hour data from last Thursday. There were some interesting results, and when the Probit analysis was run, it said that our LD50 was 0.08 microliters/Liter. This is similar to the results we received last time (0.05 microliters/Liter). We then decided to start our first outdoor trial and scaled up to a “mesocosm” (5 L of larval water rather than 250 mL). We put 5 mL of 1 microliter/Liter solution into it. I took note that most of the larvae were dead within the first 24 hours, and they were all dead after 72 hours

Week 3: (6/17-6/21)

  Black Fly This Week:  I had an exciting week working with black flies! I collected my first independent samples from three different sites in order to determine if we needed to treat the river (spoiler alert: we did). I am still working on correctly identifying black flies larvae in comparison to other species, but I was able to identify black flies at 2 of the 3 sites I went to. I sorted through the samples afterwards. I saw what they looked like under the microscope, which was not only interesting but also helpful. Attached are some pictures!! Once it was determined that the river needed to be treated, I went out into the field to see how it was done. We went to seven different sites and treated with BTI.  Bioassay Update & Mosquito Work This Week:  Since I completed my first bioassay last week, we started to analyze the results of it! Although we still need to run duplicate bioassays, I learned some preliminary results in order to adjust for the future. We received fiv

Week 2: (6/10-6/14)

  Natular SC Bioassay One:  This week, I set up and completed our first experimental Bioassay! I used 250mL of the treatments in four cups for each of the 8 treatments (ranging from 0.01 microliters/Liter to 1 microliter/Liter of Natular SC) as well as a control which used larval water. I placed about 15 larvae in each cup which totaled to be 504 larvae. I recorded results over each of the five days this week with Monday being 0 hours and Friday being 96 hours since the start. Each day I counted how many larvae were alive and how many were dead, then added up the total for each cup as well as the all together. To the right is a picture of the set up! Below is Total column of the data sheet created:  Total Time (hours) since start of experiment  Alive Dead Total  0 504 0 504 24 347 157 504 28 277 227 504 72 235 269 504 96 207 297 504 I enjoyed completing my first complete bioassay, and I am excited to work on a few more over the course of the summer. I also am interested in the statis

Week 1 (6/3-6/7):

  During my first week as an intern, I got a taste for both lab and field work!! In the lab, I first learned common types of mosquitoes including the Asian Tiger mosquito or Aedes Albopictus, which we will be working with over the course of the summer. I worked with “Dr. Jack” Petersen a lot this week, and he was incredibly informative on all things mosquitoes! I was very excited to get started, and we set up a new infirmary as well as a sample bioassay on the first day. I already feel more confident in my ability to determine different life stages of the mosquitoes. Additionally, much of the first week was defining the first bioassay we are going to be running with Natular SC, which is a larvicide that would be new to New Jersey.  Our research question is how many microliters of Natular SC are needed to treat 100 L of larvae? We hope to answer the question through a regression and probit analysis of the data. We then decided 8 different concentrations to use for 8 different treatments

Welcome to my Internship Blog!

 Hello! My name is Abby Golembiewski, and I am a biochemistry student at Villanova University. This summer I will be interning with the Hunterdon County Mosquito and Vector Control Program in Flemington, New Jersey. I am excited to gain experience in field and laboratory work while studying black flies and Asian tiger mosquitoes!!